| biooe# | Size | Amount | Concentration | Price |
| M0505S | 200 reactions (50 µl) | 1 x 5 ml | 2 X | $51.00 |
| M0505M | 1000 reactions (50 µl) | 5 x 5 ml | 2 X | $257.00 |
High-Fidelity DNA Polymerase Master Mix is a convenient, ready-to-use 2X mixture of OE High-Fidelity DNA Polymerase, buffer, magnesium chloride, and dNTPs.
OE High-Fidelity DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of OE High-Fidelity DNA Polymerase is 80 times higher than that of Taq DNA polymerase. The optimized Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized Reaction Buffer, OE High-Fidelity DNA Polymerase is capable to amplify most templates, such as longer targets (up to 25 kb from lambda DNA) and that contain GC-rich sequences.
FEATURES
- High fidelity: >80x Taq Fidelity
- 3’→5’ proofreading exonuclease activity
- Long range capacity: 18 kb for gDNA and 25 kb for λ DNA
- Good coverage on DNA templates with low to high GC content
Applications
- High-fidelity PCR
- Cloning
- Template generation for sequencing
- GC-rich PCR
- Long-range PCR (up to 25 kb)
- Mutagenesis
- High-throughput PCR
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