| biooe# | Size | Amount | Concentration | Price |
| M0504S | 100 reactions (50 µl) | 1 x 1 ml | 5 X | $17.00 |
| M0504M | 1000 reactions (50 µl) | 1 x 10 ml | 5 X | $170.00 |
Multiplex PCR Master Mix is a perfect choice for endpoint multiplex PCR. The unique buffer composition is optimized for robust simultaneous amplification of 15 or more targets from 50 bp – 2 kb in a single reaction.
The Master Mix contains pure Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized Multiplex PCR buffer; thus, only template, PCR primers and PCR-grade water are added. The enhancers included in the mix enable efficient amplification of low abundant or GC-rich templates.
Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. The hot-start efficiently minimizes primer–dimers and mispriming.
Features
- Excellent performance and robustness in multiplex PCR
- Optimized Master Mix for minimal hands-on and fast setup
- Hot-start for highest sensitivity and specificity
- Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
Applications
- Fast and high-throughput multiplex PCR
- Parallel detection of multiple targets in a single assay
- Gene expression analysis, diagnostic and forensic genotyping
- Mutation analysis
- Gene deletion analysis
- Template quantification
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