| biooe# | Component Name | Amount | Concentration | Price |
| M0501S | Taq DNA Polymerase | 1 x 0.2 ml | 2,000U/ml | $10 |
| Taq Reaction Buffer | 1 x 1 ml | 10 X | ||
| M0501M | Taq DNA Polymerase | 1 x 1 ml | 2,000U/ml | $38 |
| Taq Reaction Buffer | 1 x 10 ml | 10 X | ||
| M0501L | Taq DNA Polymerase | 1 x 10 ml | 2,000U/ml | $377 |
| Taq Reaction Buffer | 1 x 100 ml | 10 X |
Description
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity；It synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa.
FEATURES
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Robust and reliable reactions
- Tolerates a wide range of templates
- Amplification of PCR products up to 5 kb in size
Applications
Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, cycle sequencing,Primer Extension,Microarray Analysis,and Colony PCR.
Source
Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Heat Inactivation
No
5′ – 3′ Exonuclease
Yes
3′ – 5′ Exonuclease
No
Strand Displacement
No
Resulting Ends
Single-base 3´ Overhangs
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